Active Recombinant Human Sema Domain, Immunoglobulin Domain(Ig), Transmembrane Domain(TM) And Short Cytoplasmic Domain,(semaphorin) 4A, His-tagged

• Cat.No.: SEMA4A-7022H
• Product Overview: A DNA sequence encoding the human SEMA4A extracellular domain ( Met 1 - His 683 ) was expressed, with a polyhistidine tag at the C-terminus. It consists of 663 amino acids and predictes a molecular mass of 73.4 kDa.

Specification

• Description: SEMA4A is a single-pass type I membrane protein which belongs to the semaphorin family. It inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons. SEMA4A contains one Ig-like C2-type (immunoglobulin-like) domain, one PSI domain and one Sema domain.
• Source: Human Cells
• Species: Human
• Tag: His
• Predicted N Terminal: Gly 32
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
• Bio-activity: Measured by its ability to bind biotinylated mouse SEMA4D in a functional ELISA.
• Molecular Mass: The recombinant human SEMA4A consists of 663 amino acids and predictes a molecular mass of 73.4 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh SEMA4A is approximately 75-80 kDa.
• Protein length: 663aa
• Endotoxin: 1.0 eu/μg of the protein as determined by the lal
• Purity: >96 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70 °C.
• Storage: Store it under sterile conditions at -70°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: SEMA4A sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4A [ Homo sapiens ]
• Official Symbol: SEMA4A
• Synonyms: SEMA4A; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4A; SEMAB; semaphorin-4A; FLJ12287; SemB; sema B; semaphorin-B; RP35; SEMB; CORD10; RP11-54H19.2
• Gene ID: 64218
• mRNA Refseq: NM_001193300
• Protein Refseq: NP_001180229
• MIM: 607292
• UniProt ID: Q9H3S1
• Chromosome Location: 1q22
• Pathway: Axon guidance, organism-specific biosystem; Axon guidance, conserved biosystem; Axon guidance, organism-specific biosystem; Developmental Biology, organism-specific biosystem; Other semaphorin interactions, organism-specific biosystem; Semaphorin interactions, organism-specific biosystem
• Function: receptor activity