Active Recombinant Mouse Spp1 Protein, His-tagged

• Cat.No.: Spp1-01M
• Product Overview: Recombinant mouse Osteopontin (17-294aa), fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Specification

• Description: Major non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
• Source: HEK293
• Species: Mouse
• Tag: His
• Form: Liquid
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. When cells are added to mouse SPP1 coated plates. The ED50 range ≤ 1.5 μg/mL.
• Molecular Mass: 31.8 kDa (287aa)
• Protein length: 17-294aa
• AA Sequence: LPVKVTDSGSSEEKLYSLHPDPIATWLVPDPSQKQNLLAPQNAVSSEEKDDFKQETLPSNSNESHDHMDDDDDDDDDDGDHAESEDSVDSDESDESHHSDESDETVTASTQADTFTPIVPTVDVPNGRGDSLAYGLRSKSRSFQVSDEQYPDATDEDLTSHMKSGESKESLDVIPVAQLLSMPSDQDNNGKGSHESSQLDEPSLETHRLEHSKESQESADQSDVIDSQASSKASLEHQSHKFHSHKDKLVLDPKSKEDDRYLKFRISHELESSSSEVN
• Purity: > 90% by SDS-PAGE
• Unit Definition: < 1 EU/μg of protein (determined by LAL method)
• Applications: SDS-PAGE, Bioactivity
• Storage: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/mL (determined by Absorbance at 280nm)
• Storage Buffer: Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Gene Information

• Gene Name: Spp1 secreted phosphoprotein 1 [ Mus musculus (house mouse) ]
• Official Symbol: Spp1
• Synonyms: Spp1; secreted phosphoprotein 1; OP; 2AR; Bsp; Eta; Opn; Ric; BNSP; BSPI; Opnl; Apl-1; ETA-1; Spp-1; osteopontin; 44 kDa bone phosphoprotein; bone sialoprotein 1; calcium oxalate crystal growth inhibitor protein; early T-lymphocyte activation 1 protein; minopontin; osteopontin-like protein
• Gene ID: 20750
• mRNA Refseq: NM_009263
• Protein Refseq: NP_033289
• UniProt ID: P10923