Recombinant Human Ras Homolog Gene Family, Member B, His-tagged

• Cat.No.: RHOB-3529H
• Product Overview: Recombinant human RhoB protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques (1-193aa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: Rho-related GTP-binding protein RhoB, also known asRhoB,is a member of the Rho GTP-binding protein family. RhoB mediates apoptosis in neoplastically transformed cells after DNA damage. It plays a negative role in tumorigenesis as deletion causes tumor formation.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT,20% glycerol, 0.2mM NaCl
• Molecular Weight: 23.9kDa (213aa), confirmed by MALDI-TOF(Molecular weight on SDS-PAGE will appear higher)
• Purity: > 90% by SDS - PAGE
• Concentration: 0.25mg/ml (determined by Bradford assay)
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MAAIRKKLVV VGDGACGKTC LLIVFSKDEF PEVYVPTVFE NYVADIEVDG KQVELALWDT AGQEDYDRLR PLSYPDTDVI LMCFSVDSPD SLENIPEKWV PEVKHFCPNV PIILVANKKD LRSDEHVRTE LARMKQEPVR TDDGRAMAVR IQAYDYLECS AKTKEGVREV FETATRAALQ KRYGSQNGCI NCC
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: RHOB ras homolog gene family, member B [ Homo sapiens ]
• Synonyms: RHOB; ARH6; ARHB; RHOH6; MST081; MSTP081; rho-related GTP-binding protein RhoB; h6; oncogene RHO H6; rho cDNA clone 6; Aplysia RAS-related homolog 6
• Gene ID: 388
• mRNA Refseq: NM_004040
• Protein Refseq: NP_004031
• MIM: 165370
• UniProt ID: P62745
• Chromosome Location: 2p24
• Function: GDP binding; GTP binding; GTPase activity; nucleotide binding; protein binding