Recombinant Mouse Rhob Protein, MYC/DDK-tagged

• Cat.No.: Rhob-642M
• Product Overview: Purified recombinant protein of full-length mouse ras homolog family member B (Rhob), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Mediates apoptosis in neoplastically transformed cells after DNA damage. Not essential for development but affects cell adhesion and growth factor signaling in transformed cells. Plays a negative role in tumorigenesis as deletion causes tumor formation. Involved in intracellular protein trafficking of a number of proteins. Targets PKN1 to endosomes and is involved in trafficking of the EGF receptor from late endosomes to lysosomes. Also required for stability and nuclear trafficking of AKT1/AKT which promotes endothelial cell survival during vascular development. Serves as a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Required for genotoxic stress-induced cell death in breast cancer cells.
• Molecular Mass: 22.1 kDa
• Purity: >80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Rhob ras homolog family member B [ Mus musculus (house mouse) ]
• Official Symbol: Rhob
• Synonyms: Rhob; ras homolog family member B; Arh6; Arhb; AA017882; rho-related GTP-binding protein RhoB; aplysia ras-related homolog B; ras homolog B; ras homolog gene family, member AB; ras homolog gene family, member B
• Gene ID: 11852
• mRNA Refseq: NM_007483
• Protein Refseq: NP_031509
• UniProt ID: P62746