Recombinant Human Phosphoglycerate Mutase 1 (Brain), His-tagged

• Cat.No.: PGAM1-2786H
• Product Overview: Recombinant human PGAM protein (1-254aa), fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Cat. No.: PGAM1-2786H
• Description: PGAM1 belongs to the phosphoglycerate mutase family. This protein is important components of glucose and 2,3-BPGA (2,3-bisphosphoglycerate) metabolism and catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The PGAM is a dimeric enzyme containing, in different tissues, different proportions of a slow-migrating muscle (MM) isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB). Mutations in this protein cause muscle phosphoglycerate mutase eficiency, also known as glycogen storage disease X.
• Source: E.coli.
• Sequences: MGSSHHHHHH SSGLVPRGSH MAAYKLVLIR HGESAWNLEN RFSGWYDADL SPAGHEEAKR GGQALRDAGY EFDICFTSVQ KRAIRTLWTV LDAIDQMWLP VVRTWRLNER HYGGLTGLNK AETAAKHGEA QVKIWRRSYD VPPPPMEPDH PFYSNISKDR RYADLTEDQL PSCESLKDTI ARALPFWNEE IVPQIKEGKR VLIAAHGNSL RGIVKHLEGL SEEAIMELNL PTGIPIVYEL DKNLKPIKPM QFLGDEETVR KAMEAVAAQG KAKK.
• Form: Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 0.1M Nacl, 1mM DTT, 10% glycerol.
• Molecular Weight: 30.9kDa (274aa), confirmed by MALDI-TOF.
• Purity: > 90% by SDS – PAGE.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Protein length: 1-254 a.a.

Gene Information

• Gene Name: PGAM1 phosphoglycerate mutase 1 (brain) [ Homo sapiens ]
• Synonyms:
  PGAM1; phosphoglycerate mutase 1 (brain); BPG-dependent PGAM 1; phosphoglycerate mutase 1; phosphoglycerate mutase A, nonmuscle form; phosphoglycerate mutase isozyme B; EC 3.1.3.13; EC 5.4.2.1; EC 5.4.2.4
• Gene ID: 5223
• mRNA Refseq: NM_002629
• Protein Refseq: NP_002620
• MIM: 172250
• UniProt ID: P18669
• Chromosome Location: 10q25.3
• Pathway: Glycolysis / Gluconeogenesis; Metabolic pathways
• Function: 2,3-bisphospho-D-glycerate 2-phosphohydrolase activity;bisphosphoglycerate mutase activity;hydrolase activity;isomerase activity;phosphoglycerate mutase activity; protein kinase binding