Recombinant Human NEK3, GST-tagged, Active

• Cat.No.: NEK3-369H
• Product Overview: Recombinant full-length human NEK3 was expressed bybaculovirusinSf9 insect cellsusing an N-terminal GST tag. MW=86 kDa.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Description: NEK3 is a member of the NEK family of protein kinases that share high amino acid homology with NIMA (never in mitosis gene a). NEK3 mRNA is detected in all the proliferating cell lines with the amount not changing during the cell cycle. During Prolactin receptor signaling, VAV2 is phosphorylated and activated by NEK3. Overexpression of NEK3 in Chinese hamster ovary cells increases cytoskeletal re-organization in response to Prolactin while downregulation of NEK3 expression by siRNA block these effects. Prolactin also stimulates interaction between NEK3 and paxillin leading to increased paxillin phosphorylation, Analysis of breast tissue microarrays show a significant up-regulation of NEK3 expression in malignant versus normal specimens.
• Sequence: Full-length.
• Applications: Kinase Assay, Western Blot.
• Storage And Stability: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Gene Information

• Gene Name: NEK3 NIMA (never in mitosis gene a)-related kinase 3 [ Homo sapiens ]
• Synonyms: NEK3; NIMA (never in mitosis gene a)-related kinase 3; HSPK36; MGC29949; NIMA-related kinase 3; glycogen synthase A kinase; hydroxyalkyl-protein kinase; phosphorylase B kinase kinase; serine/threonine-protein kinase NEK3; EC 2.7.11.1
• Gene ID: 4752
• mRNA Refseq: NM_001146099
• Protein Refseq: NP_001139571
• MIM: 604044
• UniProt ID: P51956
• Chromosome Location: 13q14.13
• Function: ATP binding; magnesium ion binding; nucleotide binding; protein phosphatase binding; protein serine/threonine kinase activity; transferase activity