Active Recombinant Full Length Human NEK3 Protein, GST-tagged

• Cat.No.: NEK3-7074HF
• Product Overview: Full-length human NEK3 [1-506(end) amino acids of accession number NP_002489.1] was expressed as N-terminal GST-fusion protein (84 kDa) using baculovirus expression system. GST-NEK3 was purified by using glutathione sepharose chromatography.

Specification

• Source: Insect (sf21)
• Species: Human
• Tag: GST
• Form: 50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
• Bio-activity: The activity was determined by ELISA. The enzyme was incubated with biotinylated substrate protein, and after stopping kinase reaction by EDTA, the reaction solution was transferred into streptavidin- coated plate. Phosphorylation was detected by anti-phospho antibody and HRP-labeled anti-rabbit IgG.
• Protein length: 1-506
• Purity: 91%
• Applications: SDS-PAGE
• Storage: Store at -80C. Avoid repeating freeze-thaws.

Gene Information

• Gene Name: NIMA (never in mitosis gene a)-related kinase 3
• Official Symbol: NEK3
• Synonyms: NEK3;HSPK36;MGC29949
• Protein Refseq: NP_002489.1
• UniProt ID: P51956