Recombinant Human NEFM protein, His-tagged

• Cat.No.: NEFM-6816H
• Product Overview: Recombinant Human NEFM aa. (Leu105~Gln287) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: 26kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Leu105~Gln287
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 5.5.
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200ug/mL
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% Trehalose.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.

Gene Information

• Gene Name: NEFM neurofilament medium [ Homo sapiens (human) ]
• Official Symbol: NEFM
• Synonyms: NFM; NEF3; NF-M; 160 kDa neurofilament protein; neurofilament 3; neurofilament triplet M protein; neurofilament, medium polypeptide 150kDa; neurofilament-3 (150 kD medium); neurofilament medium polypeptide
• Gene ID: 4741
• mRNA Refseq: NM_001105541.1
• Protein Refseq: NP_001099011.1
• UniProt ID: Q9UK51