Recombinant Human NEFM Protein, His-tagged

• Cat.No.: NEFM-2485H
• Product Overview: Recombinant Human NEFM Protein (Met1-Ser358) with a N-His tag was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Met1-Ser358
• Description: Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 44.5 kDa
  Accurate Molecular Mass: 44 kDa
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 95%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in PBS or others.

Gene Information

• Gene Name: NEFM neurofilament, medium polypeptide [ Homo sapiens (human) ]
• Official Symbol: NEFM
• Synonyms: NEFM; neurofilament, medium polypeptide; NEF3, neurofilament, medium polypeptide 150kDa; neurofilament medium polypeptide; NF M; NFM; neurofilament 3; 160 kDa neurofilament protein; neurofilament triplet M protein; neurofilament-3 (150 kD medium); neurofilament, medium polypeptide 150kDa; NEF3; NF-M
• Gene ID: 4741
• mRNA Refseq: NM_001105541
• Protein Refseq: NP_001099011
• MIM: 162250
• UniProt ID: P07197