Recombinant Human ME2

• Cat.No.: ME2-644H
• Product Overview: Recombinant Human ME2 produced in E. coli is approximately 63.5 kDa, a single non-glycosylated polypeptide chain containing 567 amino acids.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: NAD-dependent malic enzyme (ME2), mitochondrial is a protein that in humans is encoded by the ME2 gene. This gene encodes a mitochondrial NAD-dependent malic enzyme, a homotetrameric protein, which catalyzes the oxidative decarboxylation of malate to pyruvate. Three different isoforms of ME are known to be in mammalian tissues: a strictly cytosolic NADP+-dependent enzyme, an NADP+-dependent mitochondriail isoform, and a mitochondrial isoenzyme that is able to use both NAD+ and NADP+ but is more effective with NAD+. The mammalian isoforms size is about 62-64 kDa. A native size of 240,000 Da proposes a tetrameric structure for the active enzyme.
• Form: Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
• Molecular Mass: 63.5 kDa
• AA Sequence: MLHIKEKGKP LMLNPRTNKG MAFTLQERQM LGLQGLLPPK IETQDIQALR FHRNLKKMTS PLEKYIYIMG IQERNEKLFY RILQDDIESL MPIVYTPTVG LACSQYGHIF RRPKGLFISI SDRGHVRSIV DNWPENHVKA VVVTDGERIL GLGDLGVYGM GIPVGKLCLY TACAGIRPDR CLPVCIDVGT DNIALLKDPF YMGLYQKRDR TQQYDDLIDE FMKAITDRYG
• Endotoxin: Less than 1 EU/μg of rHuME2 as determined by LAL method.
• Purity: >95% by SDS-PAGE and HPLC analyses.
• Usage: This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
• Storage: This lyophilized preparation is stable at 2-8 centigrade, but should be kept at -20 centigrade for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 centigrade. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 centigrade to -70 centigrade. Avoid repeated freeze/thaw cycles.
• Reconstitution: We recommend that this vial be briefy centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 centigrade. Further dilutions should be made in appropriate buffered solutions.

Gene Information

• Gene Name: ME2 malic enzyme 2, NAD(+)-dependent, mitochondrial [ Homo sapiens ]
• Official Symbol: ME2
• Synonyms: ME2; malic enzyme 2, NAD(+)-dependent, mitochondrial; NAD-dependent malic enzyme, mitochondrial; NAD-ME; malate dehydrogenase; pyruvic-malic carboxylase; ODS1
• Gene ID: 4200
• mRNA Refseq: NM_001168335
• Protein Refseq: NP_001161807
• MIM: 154270
• UniProt ID: P23368
• Chromosome Location: 18q21
• Pathway: Pyruvate metabolism, organism-specific biosystem; Pyruvate metabolism, conserved biosystem; gluconeogenesis I, organism-specific biosystem
• Function: NAD binding; electron carrier activity; malate dehydrogenase (decarboxylating) activity; malate dehydrogenase (oxaloacetate-decarboxylating) activity; metal ion binding; oxidoreductase activity