Recombinant Human KL 293 Cell Lysate

• Cat.No.: KL-4936HCL

Specification

• Description: Antigen standard for klotho (KL) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
• Tag: Non

Gene Information

• Gene Name: KL klotho [ Homo sapiens ]
• Official Symbol: KL
• Synonyms: KL; klotho
• Gene ID: 9365
• mRNA Refseq: NM_004795
• Protein Refseq: NP_004786
• UniProt ID: Q9UEF7
• Chromosome Location: 13q12
• Pathway: Ascorbate biosynthesis, animals, glucose-1P =>ascorbate, organism-specific biosystem; Ascorbate biosynthesis, animals, glucose-1P => ascorbate, conserved biosystem; Downstream signaling of activated FGFR, organism-specific biosystem; Endocrine and other factor-regulated calcium reabsorption, organism-specific biosystem; Endocrine and other factor-regulated calcium reabsorption, conserved biosystem
• Function: beta-glucosidase activity; beta-glucuronidase activity; cation binding; fibroblast growth factor binding; fibroblast growth factor receptor binding; hormone activity; signal transducer activity; vitamin D binding

Reviews

01/31/2021

The KL protein's reliability and ease of use have made it an essential component of my structural investigations.

01/22/2019

Its ability to preserve structural integrity and facilitate high-resolution imaging has greatly enhanced my understanding of protein complexes and their interactions.

07/22/2016

Furthermore, the KL protein has demonstrated great utility in protein electron microscopy structure analysis.

Q&As

How does KL protein affect cognitive function?

KL protein may have a neuroprotective role and is being studied for its potential in preserving cognitive function and preventing neurodegenerative diseases.

Are there any KL protein-based therapies available for clinical use?

Currently, there are no FDA-approved KL protein-based therapies, but research in this area is ongoing.

Can KL protein supplementation slow down the aging process?

While research suggests a connection between KL protein and aging, it is not yet clear if KL protein supplementation can effectively slow down the aging process in humans.

How is KL protein measured in clinical settings?

KL protein levels can be measured in blood and urine samples using various laboratory techniques.

Are there any clinical trials exploring KL protein-based therapies?

Yes, several clinical trials are investigating the potential therapeutic benefits of KL protein supplementation in kidney disease and other disorders.