Recombinant Human KDM4A 293 Cell Lysate

• Cat.No.: KDM4A-4996HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for lysine (K)-specific demethylase 4A (KDM4A) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: KDM4A lysine (K)-specific demethylase 4A [ Homo sapiens ]
• Official Symbol: KDM4A
• Synonyms: KDM4A; lysine (K)-specific demethylase 4A; JMJD2, JMJD2A, jumonji domain containing 2 , jumonji domain containing 2A; lysine-specific demethylase 4A; JHDM3A; jumonji C domain containing histone demethylase 3A; KIAA0677; jumonji domain containing 2; jumonji domain containing 2A; jumonji domain-containing protein 2A; jumonji C domain-containing histone demethylase 3A; jmjC domain-containing histone demethylation protein 3A; JMJD2; JMJD2A
• Gene ID: 9682
• mRNA Refseq: NM_014663
• Protein Refseq: NP_055478
• MIM: 609764
• UniProt ID: O75164
• Chromosome Location: 1p34.1
• Function: histone demethylase activity (H3-K36 specific); metal ion binding; oxidoreductase activity; oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; protein binding; zinc ion binding