Recombinant Human HSBP1 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: HSBP1-824H
• Product Overview: HSBP1 MS Standard C13 and N15-labeled recombinant protein (NP_001528) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: The heat-shock response is elicited by exposure of cells to thermal and chemical stress and through the activation of HSFs (heat shock factors) results in the elevated expression of heat-shock induced genes. Heat shock factor binding protein 1 (HSBP1), is a 76-amino-acid protein that binds to heat shock factor 1(HSF1), which is a transcription factor involved in the HS response. During HS response, HSF1 undergoes conformational transition from an inert non-DNA-binding monomer to active functional trimers. HSBP1 is nuclear-localized and interacts with the active trimeric state of HSF1 to negatively regulate HSF1 DNA-binding activity. Overexpression of HSBP1 in mammalian cells represses the transactivation activity of HSF1. When overexpressed in C.elegans HSBP1 has severe effects on survival of the animals after thermal and chemical stress consistent with a role of HSBP1 as a negative regulator of heat shock response.
• Source: HEK293
• Species: Human
• Tag: Myc&DDK
• Molecular Mass: 8.5 kDa
• AA Sequence: MAETDPKTVQDLTSVVQTLLQQMQDKFQTMSDQIIGRIDDMSSRIDDLEKNIADLMTQAGVEELESENKIPATQKSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: HSBP1 heat shock factor binding protein 1 [ Homo sapiens (human) ]
• Official Symbol: HSBP1
• Synonyms: HSBP1; heat shock factor binding protein 1; heat shock factor-binding protein 1; nasopharyngeal carcinoma-associated antigen 13; NPC-A-13; DKFZp686D1664; DKFZp686O24200
• Gene ID: 3281
• mRNA Refseq: NM_001537
• Protein Refseq: NP_001528
• MIM: 604553
• UniProt ID: O75506