Recombinant Human HMGB1 protein(Met 1-Glu 215), His-tagged

• Cat.No.: HMGB1-3827H
• Product Overview: Recombinant Human HMGB1 protein(NP_002119.1) (Met 1-Glu 215) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus.

Specification

• Product Overview: Recombinant Human HMGB1 protein(NP_002119.1) (Met 1-Glu 215) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus.
• Source: HEK293
• Species: Human
• Tag: His
• Protein length: Met 1-Glu 215
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized human HMGB at 2 μg/ml (100 μl/well) can bind human AGER. The EC50 of human AGER is 0.27 μg/ml.
• Molecular Mass: The recombinant human HMGB1 consists of 226 amino acids and has a predicted molecular mass of 26.3 kDa. As a result of glycosylation, the apparent molecular mass of rhHMGB1 is approximately 30-34 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: ≥ 93 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: HMGB1 high mobility group box 1 [ Homo sapiens ]
• Official Symbol: HMGB1
• Synonyms: HMGB1; high mobility group box 1; high mobility group (nonhistone chromosomal) protein 1 , high mobility group box 1 , HMG1; high mobility group protein B1; Amphoterin; DKFZp686A04236; high mobility group protein 1; HMG3; SBP 1; Sulfoglucuronyl carbohydrate binding protein; HMG-1; high-mobility group box 1; high-mobility group (nonhistone chromosomal) protein 1; HMG1; SBP-1
• Gene ID: 3146
• mRNA Refseq: NM_002128
• Protein Refseq: NP_002119
• MIM: 163905
• UniProt ID: P09429