Active Native Bovine HMGB1

• Cat.No.: HMGB1-8447B
• Product Overview: Native Bovine HMGB1 was purified from Calf Thymus.

Specification

• Species: Calf
• Source: Calf Thymus
• Tag: Non
• Description: High mobility group 1 (HMG1) is a 26 kDa highly conserved non-sequence-specific DNA-binding nuclear protein. Mammalian HMG1 has two homologous DNA-binding domains HMG boxes, A and B (each of 80–90 amino-acid residues), linked by a short basic region to an acidic C-terminal domain containing 30 consecutive Asp and Glu residues. HMG1 has been implicated in a number of fundamental biological processes including transcription, replication and recombination, in which it plays a role in manipulating DNA structure by bending, looping, compaction or unwinding, or by directly contacting with distinct cellular proteins . HMG-1 can act as a repressor, by interacting with TBP to block pre-initiation complex formation or as an activator, by facilitating the binding of various transcription factors to their cognate DNA sequences. Most recently, it was discovered that HMG-1 is a late mediator of delayed endotoxin lethality by activating downstream cytokine release.
• Form: 20 mM Tris-Cl, pH 7.9, 20% Glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA
• Bio-activity: 1 unit equals 1 ng of purified protein. 10-100 units are required for a gel mobility shift assay in a 20μl reaction to super-shift TBP-DNA complex, 50-500 units are required for a reconstituted transcription assay and 100 units are sufficient for a protein-protein interaction assay.
• AA Sequence: MGKGDPKKPR GKMSSYAFFV QTCREEHKKK HPDASVNFSE FSKKCSERWK TMSAKEKGKF EDMAKADKAR YEREMKTYIP PKGETKKKFK DPNAPKRPPS AFFLFCSEYR PKIKGEHPGL SIGDVAKKLG EMWNNTAADD KQPYEKKAAK LKEKYEKDIA AYRAKGKPDA AKKGVVKAEK SKKKKEEEED EEDEEDEEEE EDEEDEEEEE DDDDE
• Purity: >95% as determined by SDS-PAGE
• Storage: -80 °C
• Concentration: 900 μg/ml
• Shipping: Dry Ice

Gene Information

• Gene Name: HMGB1 high mobility group box 1 [ Bos taurus ]
• Official Symbol: HMGB1
• Synonyms: HMGB1; high mobility group box 1; high mobility group protein B1; HMG-1; high mobility group protein 1
• Gene ID: 282691
• mRNA Refseq: NM_176612
• Protein Refseq: NP_788785
• UniProt ID: 10103
• Chromosome Location: chromosome: 12
• Pathway: Androgen Receptor Signaling Pathway, organism-specific biosystem; Base excision repair, organism-specific biosystem; Base excision repair, conserved biosystem
• Function: DNA binding, bending; chemoattractant activity; damaged DNA binding; double-stranded DNA binding