Recombinant Human Guanylate Kinase 1, His-tagged

• Cat.No.: GUK1-2785H
• Product Overview: Recombinant human GUK1 protein (1-197aa), fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• ProteinLength: 1-197 a.a.
• Description: GUK1, also known as GMK, belongs to the guanylate kinase family. This protein exists as a monomer that catalyzes the ATP-dependent conversion of GMP to GDP, thereby playing an essential role in the recycling of GMP.
• Sequences: MGSSHHHHHH SSGLVPRGSH MSGPRPVVLS GPSGAGKSTL LKRLLQEHSG IFGFSVSHTT RNPRPGEENG KDYYFVTREV MQRDIAAGDF IEHAEFSGNL YGTSKVAVQA VQAMNRICVL DVDLQGVRNI KATDLRPIYI SVQPPSLHVL EQRLRQRNTE TEESLVKRLA AAQADMESSK EPGLFDVVII NDSLDQAYAE LKEALSEEIK KAQRTGA.
• Form: Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 0.1M Nacl, 1mM DTT, 10% glycerol.
• Molecular Weight: 23.9 kDa (217aa), confirmed by MALDI-TOF.
• Purity: > 90% by SDS – PAGE.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: GUK1 guanylate kinase 1 [ Homo sapiens ]
• Synonyms:
  GUK1; guanylate kinase 1; GMP kinase; OTTHUMP00000037738; OTTHUMP00000037739; OTTHUMP00000037740; OTTHUMP00000037741; OTTHUMP00000037742; OTTHUMP00000037743; OTTHUMP00000037746; OTTHUMP00000037747; OTTHUMP00000037748; OTTHUMP00000037750; OTTHUMP00000037751; guanylate kinase
• Gene ID: 2987
• mRNA Refseq: NM_000858
• Protein Refseq: NP_000849
• MIM: 139270
• UniProt ID: Q16774
• Chromosome Location: 1q32-q41
• Pathway: Metabolic pathways; Purine metabolism; Metabolism of nucleotides
• Function: ATP binding;guanylate kinase activity;kinase activity;nucleotide binding;transferase activity