Recombinant Human ENOPH1 protein, His-tagged

• Cat.No.: ENOPH1-3457H
• Product Overview: Recombinant Human ENOPH1(1-261aa) fused with His tag at N-terminal was expressed in E. coli.

Specification

• Description: Enolase-phosphatase E1, also known as ENOPH1, is a member of the MasA family of the HAD (halo-acid dehalogenase)-like hydrolase superfamily. ENOPH1 is a bifunctional enzyme, exhibiting both phosphatase and atypical enolase activities. ENOPH1 plays an important role in the ubiquitous methionine salvage pathway, a biochemical pathway found in all organisms that regulate methionine levels in the cell.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 100mM NaCl
• Molecular Mass: 31 kDa (281aa), confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSHGAVPIPAASG NGVDDLQQMI QAVVDNVCWQ MSLDRKTTAL KQLQGHMWRA AFTAGRMKAE FFADVVPAVR KWREAGMKVY IYSSGSVEAQKLLFGHSTEG DILELVDGHF DTKIGHKVES ESYRKIADSI GCSTNNILFL TDVTREASAA EEADVHVAVV VRPGNAGLTD DEKTYYSLITMVVLSVPAEV TVILLDIEGT TTPIAFVKDI LFPYIEENVK EYLQTHWEEE ECQQDVSLLR KQAEEDAHLDSFSELYLPSS T
• Purity: >90% by SDS - PAGE
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)
• Protein length: 1-261 a.a.

Gene Information

• Gene Name: ENOPH1 enolase-phosphatase 1 [ Homo sapiens ]
• Official Symbol: ENOPH1
• Synonyms: ENOPH1; enolase-phosphatase 1; enolase-phosphatase E1; acireductone synthase; E1; Enolase phosphatase E1; MASA; MASA homolog; 2,3-diketo-5-methylthio-1-phosphopentane phosphatase; MST145; FLJ12594; DKFZp586M0524
• Gene ID: 58478
• mRNA Refseq: NM_021204
• Protein Refseq: NP_067027
• UniProt ID: Q9UHY7
• Chromosome Location: 4q21.3
• Pathway: Cysteine and methionine metabolism, organism-specific biosystem; Cysteine and methionine metabolism, conserved biosystem; Metabolism, organism-specific biosystem; Metabolism of amino acids and derivatives, organism-specific biosystem; Metabolism of polyamines, organism-specific biosystem; Methionine salvage pathway, organism-specific biosystem; Methionine salvage pathway, organism-specific biosystem
• Function: 2,3-diketo-5-methylthiopentyl-1-phosphate enolase activity; 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase activity; acireductone synthase activity; acireductone synthase activity; hydrolase activity; magnesium ion binding; phosphoglycolate phosphatase activity