Recombinant Human DVL2 protein, His-tagged

• Cat.No.: DVL2-1844H
• Product Overview: Recombinant Human DVL2 aa. (Leu78~Thr250) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: This gene encodes a member of the dishevelled (dsh) protein family. The vertebrate dsh proteins have approximately 40% amino acid sequence similarity with Drosophila dsh. This gene encodes a 90-kD protein that undergoes posttranslational phosphorylation to form a 95-kD cytoplasmic protein, which may play a role in the signal transduction pathway mediated by multiple Wnt proteins. The mechanisms of dishevelled function in Wnt signaling are likely to be conserved among metazoans.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: 27kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Leu78~Thr250
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Isoelectric Point: 8.1

Gene Information

• Gene Name: DVL2 dishevelled segment polarity protein 2 [ Homo sapiens (human) ]
• Official Symbol: DVL2
• Synonyms: DVL2; dishevelled segment polarity protein 2; segment polarity protein dishevelled homolog DVL-2; dishevelled 2 (homologous to Drosophila dsh); dishevelled, dsh homolog 2
• Gene ID: 1856
• mRNA Refseq: NM_004422.2
• Protein Refseq: NP_004413.1
• UniProt ID: O14641