Recombinant Human CYP51A1, GST-tagged

• Cat.No.: CYP51A1-11794H
• Product Overview: Recombinant Human CYP51A1 protein, fused to GST-tag, was expressed in E.coli and purified by GSH-sepharose.

Specification

• Species: Human
• Source: E.coli
• Tag: GST
• ProteinLength: 146-509a.a.
• Description: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein participates in the synthesis of cholesterol by catalyzing the removal of the 14alpha-methyl group from lanosterol. Homologous genes are found in all three eukaryotic phyla, fungi, plants, and animals, suggesting that this is one of the oldest cytochrome P450 genes. Two transcript variants encoding different isoforms have been found for this gene.
• Storage: The protein is stored in PBS buffer at -20℃. Avoid repeated freezing and thawing cycles.
• Storage Buffer: 1M PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8. ) added with 100mM GSH and 1% Triton X-100,15%glycerol.

Gene Information

• Gene Name: CYP51A1 cytochrome P450, family 51, subfamily A, polypeptide 1 [ Homo sapiens ]
• Official Symbol: CYP51A1
• Synonyms: CP51; CP51A_HUMAN; CYP51; CYP51A1; CYPLI; Cytochrome P450 51; Cytochrome P450 51A1; Cytochrome P450-14DM; Cytochrome P45014DM; Cytochrome P450LI; Lanosterol 14-alpha demethylase; LDM; P450 14DM; P450L1; Sterol 14-alpha demethylase
• Gene ID: 1595
• mRNA Refseq: NM_000786.3
• Protein Refseq: NP_000777.1
• MIM: 601637
• UniProt ID: Q16850
• Chromosome Location: 7q21.2
• Pathway: Activation of Gene Expression by SREBP (SREBF), organism-specific biosystem; Biological oxidations, organism-specific biosystem; Cholesterol Biosynthesis, organism-specific biosystem; Cholesterol biosynthesis, organism-specific biosystem; Cholesterol biosynthesis, squalene 2,3-epoxide =>cholesterol, organism-specific biosystem; Cholesterol biosynthesis, squalene 2,3-epoxide =>
• Function: electron carrier activity; heme binding; iron ion binding; sterol 14-demethylase activity