Recombinant Human CYP51A1 293 Cell Lysate

• Cat.No.: CYP51A1-7100HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for cytochrome P450, family 51, subfamily A, polypeptide 1 (CYP51A1), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: CYP51A1 cytochrome P450, family 51, subfamily A, polypeptide 1 [ Homo sapiens ]
• Official Symbol: CYP51A1
• Synonyms: CP51; CP51A_HUMAN; CYP51; CYP51A1; CYPLI; Cytochrome P450 51; Cytochrome P450 51A1; Cytochrome P450-14DM; Cytochrome P45014DM; Cytochrome P450LI; Lanosterol 14-alpha demethylase; LDM; P450 14DM; P450L1; Sterol 14-alpha demethylase
• Gene ID: 1595
• mRNA Refseq: NM_000786.3
• Protein Refseq: NP_000777.1
• MIM: 601637
• UniProt ID: Q16850
• Chromosome Location: 7q21.2
• Pathway: Activation of Gene Expression by SREBP (SREBF), organism-specific biosystem; Biological oxidations, organism-specific biosystem; Cholesterol Biosynthesis, organism-specific biosystem; Cholesterol biosynthesis, organism-specific biosystem; Cholesterol biosynthesis, squalene 2,3-epoxide =>cholesterol, organism-specific biosystem; Cholesterol biosynthesis, squalene 2,3-epoxide =>
• Function: electron carrier activity; heme binding; iron ion binding; sterol 14-demethylase activity