Recombinant Human Cyclin B1, His-tagged

• Cat.No.: CCNB1-1529H
• Product Overview: Recombinant human CCNB1 (aa. 1-433) produced inE.coliis a 49.3 kDa protein. It was fused to His-Tag at N-Terminus.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: Cyclin B1 is a regulatory protein involved in mitosis. It complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.
• Purity: 95%.
• Preparation: 0.05mg/0.17 ml.
• Tested Application: E, WB, MS.
• Buffer: 10 mM Tris, pH 8.0, 0.1% Triton X-100, 0.002% NaN3, 10mM DTT.
• Species Reactivity: n/aH.
• Storage: Store at -70°C with any protein, exposing CCNB1 recombinant protein to repeated freeze/thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
• Pathways: Cell cycle; Progesterone-mediated oocyte maturation; p53 signaling pathway; Cell Cycle Checkpoints; Cell Cycle, Mitotic

Gene Information

• Gene Name: CCNB1 cyclin B1 [ Homo sapiens ]
• Synonyms: cyclin B1; CCNB; CCNB1; G2/mitotic-specific cyclin B1
• Gene ID: 891
• mRNA Refseq: NM_031966
• Protein Refseq: NP_114172
• UniProt ID: P14635
• MIM: 123836
• Chromosome Location: 5q12
• Function: protein binding