Recombinant Human CASP3 protein, His-tagged

• Cat.No.: CASP3-621H
• Product Overview: Recombinant Human CASP3 aa. (Ala183~His277) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Ala183~His277
• Description: This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.
• Form: Freeze-dried powder
• Molecular Mass: 15kDa as determined by SDS-PAGE reducing conditions
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >98%
• Characteristic: The isoelectric point is 7.7.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200µg/mL
• Storage buffer: PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and Proclin300.
• Reconstitution: Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: CASP3 caspase 3 [ Homo sapiens (human) ]
• Official Symbol: CASP3
• Synonyms: CASP3; caspase 3; CPP32; SCA-1; CPP32B; caspase-3; CASP-3; CPP-32; PARP cleavage protease; SREBP cleavage activity 1; apopain; caspase 3, apoptosis-related cysteine peptidase; caspase 3, apoptosis-related cysteine protease; cysteine protease CPP32; procaspase3; protein Yama
• Gene ID: 836
• mRNA Refseq: NM_004346.3
• Protein Refseq: NP_004337.2
• UniProt ID: P42574