Active Recombinant Mouse Casp3 protein

• Cat.No.: Casp3-1643M
• Product Overview: Recombinant Mouse Casp3 protein was expressed in E. coli.

Specification

• Species: Mouse
• Source: E.coli
• Tag: Non
• Bio-activity: 300000 units/mg
• Molecular Mass: Of large (17 kD) and small (11 kD) subunits
• Unit Definition: One unit of the recombinant caspase-3 is the enzyme activity that cleaves 1 nmol of the caspase substrate DEVD-pNA (pNA: pnitroanaline) per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.
• Applications: Active caspase-3 is useful in studying enzyme regulation, determining target substrates, screening caspase inhibitors, or as a positive control in caspase activity assays. We recommend using 1 unit/assay for analyzing caspase activity.
• Notes: Centrifuge the vial prior to opening.
• Storage: The lyophilized caspase-3 is stable for 1 year at –70 centigrade. Following reconstitution in PBS, the enzyme should be aliquoted and immediately stored at –70 centigrade. Avoid multiple freeze/thaw cycles as activity might decrease.
• Reconstitution: Reconstitute to 1 unit per µl in PBS containing 15% glycerol

Gene Information

• Gene Name: Casp3 caspase 3 [ Mus musculus ]
• Official Symbol: Casp3
• Synonyms: CASP3; caspase 3; caspase-3; SCA-1; CASP-3; CPP-32; protein Yama; cysteine protease CPP32; SREBP cleavage activity 1; caspase 3, apoptosis related cysteine protease; CC3; AC-3; Lice; Yama; mldy; CPP32; Apopain; Caspase-3; A830040C14Rik
• Gene ID: 12367
• mRNA Refseq: NM_009810
• Protein Refseq: NP_033940
• Pathway: Activation of DNA fragmentation factor, organism-specific biosystem; Activation of caspases through apoptosome-mediated cleavage, organism-specific biosystem; Alpha6-Beta4 Integrin Signaling Pathway, organism-specific biosystem
• Function: aspartic-type endopeptidase activity; cyclin-dependent protein kinase inhibitor activity; cysteine-type endopeptidase activity; cysteine-type peptidase activity; cysteine-type peptidase activity