Recombinant Human CASP3, His tagged

• Cat.No.: CASP3-342H
• Product Overview: Recombinant Human CASP3 mature form (Q5IS54) (Ser29-His277), fused with a polyhistidine tag at the C-terminus, was produced in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• ProteinLength: 29-277 a.a.
• Predicted N Terminal: Met
• Form: Lyophilized from sterile 20mM HEPES, 100mM NaCl, 1mM EDTA, 10% Sucrose, 0.1% chaps, pH 7.5.Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization.
• Molecular Mass: The recombinant human caspase3 consists of 259 amino acids and predicts a molecular mass of 29.9 KDa. It migrates as an approximately 17 and 15 KDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 eu per μg of the protein as determined by the LAL method.
• Purity: (48.4+48.1) % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70oC.
• Storage: Store it under sterile conditions at -20oC~-70oC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: CASP3 caspase 3, apoptosis-related cysteine peptidase [ Homo sapiens ]
• Official Symbol: CASP3
• Gene ID: 836
• mRNA Refseq: NM_004346
• Protein Refseq: NP_004337
• MIM: 600636
• UniProt ID: P42574
• Chromosome Location: 4q34