Recombinant Human Angiopoietin 4

• Cat.No.: ANGPT4-1587H

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: Non
• Description: shares 45%, 47% and 54% aa sequence identity with human Ang-1, human Ang-2 and mouse Ang-3, respectively. Although the sequence homology is much higher between the human and mouse counterparts for Ang-1 (97%) and Ang-2 (85%), mouse Ang-3 is believed to be an ortholog of human Ang-4 based on chromosomal localization studies. Human Ang-4 is highly expressed in lung and in cultured human umbilical vein endothelial cells (HUVECs). In contrast, mouse Ang-3 is expressed multiple mouse tissues. Human Ang-4 is an agonist that can bind and activate Tie-2, a receptor tyrosine kinase with immunoglobulin and epidermal growth factor homology domains expressed primarily on endothelial cells and early hematopoietic cells. Mouse Ang-3 has been reported to be a Tie-2 antagonist. It is likely that mouse Ang-3, like Ang-2, may exert agonist or antagonist activities depending on the cell context.
• State Of Matter: Lyophilized.
• Purity: >85% by SDS PAGE and analyzed by silver stain.
• Endotoxin: <1.0 EU/µg as determined by the LAL method.
• Molecular Weight: The predicted molecular weight of Recombinant Human Ang-4 is Mr 57 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 60-100 kDa.
• Storage And Stability: This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Gene Information

• Gene Name: ANGPT4 angiopoietin 4 [ Homo sapiens ]
• Synonyms: angiopoietin 4; AGP4; ANG4; ANG-3; MGC138181; MGC138183; ANGPT4; angiopoietin-3; dJ824F16.2 (angiopoietin 4); ANG-4; ANG3; OTTHUMP00000029947
• Gene ID: 51378
• mRNA Refseq: NM_015985
• Protein Refseq: NP_057069
• MIM: 603705
• UniProt ID: Q9Y264
• Chromosome Location: 20p13
• Pathway: Hemostasis; Signaling in Immune system
• Function: transmembrane receptor protein tyrosine kinase activator activity; receptor binding