Recombinant Full Length Human transcription factor EB Protein, T7&His&TEV tagged

• Cat.No.: TFEB-12H
• Product Overview: Full-length human TFEB (475 aa, Isoform-1. derived from BC032448) gene was constructed with codon optimized gene synthesis technology, and fusion with 30 aa N-terminal T7 / His / TEV cleavage site Tag. It was expressed in E.coli as inclusion bodies.

Specification

• Species: Human
• Source: E.coli
• Tag: His&T7&TEV
• Description: Enables DNA-binding transcription factor activity; enzyme binding activity; and transcription cis-regulatory region binding activity. Involved in several processes, including antibacterial innate immune response; cellular response to amino acid starvation; and positive regulation of transcription by RNA polymerase II. Located in cytosol and lysosomal membrane. Is active in nucleus.
• Tag: N-T7&His&TEV
• Form: Sterile-filtered
• AA Sequence: MASMTGGQQMGRGHHHHHHENLYFQGGFASRIGLRMQLMREQAQQEEQRERMQQQAVMHYMQQQQQQQQQQLGGPPTPAINTPVHFQSPPPVPGEVLKVQSYLENPTSYHLQQSQHQKVREYLSETYGNKFAAHISPAQGSPKPPPAASPGVRAGHVLSSSAGNSAPNSPMAMLHIGSNPERELDDVIDNIMRLDDVLGYINPEMQMPNTLPLSSSHLNVYSSDPQVTASLVGVTSSSCPADLTQKRELTDAESRALAKERQKKDNHNLIERRRRFNINDRIKELGMLIPKANDLDVRWNKGTILKASVDYIRRMQKDLQKSRELENHSRRLEMTNKQLWLRIQELEMQARVHGLPTTSPSGMNMAELAQQVVKQELPSEEGPGEALMLGAEVPDPEPLPALPPQAPLPLPTQPPSPFHHLDFSHSLSFGGREDEGPPGYPEPLAPGHGSPFPSLSKKDLDLMLLDDSLLPLASDPLLSTMSPEASKASSRRSSFSMEEGDVL
• Purity: > 90% by SDS-PAGE
• Applications: 1. May be used for in vitro TFEB mediated autophagy-lysosome activation regulation study.
  2. May be used as specific protein substrate for kinase and ubiquitin (Sumo pathway) related enzyme functional screening assays.
  3. May be used for TFEB protein-protein interaction mapping.
  4. As immunogen for specific antibody production.
• Storage: In Liquid. Keep at -80 centigrade for long term storage. Product is stable at 4 centigrade for at least 7 days.
• Storage Buffer: 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, Sucrose and DTT.
• Concentration: 1.0 mg/mL
• Reference: 1. Rushika M.Perera, et al., Transcriptional control of autophage-lysosome function drives pancreatic cancer metabolism. Nature. Vol 524, 361-365 (2015)
  2. Magini A, et al., TFEB activation promotes the recruitment of lysosomal glycohydrolases beta-hexosaminidase and beta-galactosidase to the plasma membrane. Biochem. Biophys. Res. Commun. 440 (2), 251-257 (2013)
  3. Song W, et al., TFEB regulates lysosomal proteostasis. Hum. Mol. Genet. 22 (10), 1994-2009 (2013)

Gene Information

• Gene Name: TFEB transcription factor EB [ Homo sapiens (human) ]
• Official Symbol: TFEB
• Synonyms: TFEB; transcription factor EB; TCFEB; BHLHE35; ALPHATFEB; transcription factor EB; T-cell transcription factor EB; class E basic helix-loop-helix protein 35
• Gene ID: 7942
• mRNA Refseq: NM_007162
• Protein Refseq: NP_009093
• MIM: 600744
• UniProt ID: P19484