Recombinant Cattle COL4A1 Protein, His-tagged

• Cat.No.: COL4A1-819C
• Product Overview: Recombinant Cattle COL4A1 Protein (Gly1445-Thr1669) with His tag was expressed in E. coli.

Specification

• Description: This gene encodes the alpha-1 subunit of the type IV collagens, an essential component of basement membranes. The encoded protein forms a triple helical heterotrimer comprised of two alpha-1 and one alpha-2 subunits that assembles into a type IV collagen network. This gene is located adjacent to the gene encoding alpha-2 subunit. Mice lacking both the alpha-1 and alpha-2 subunits of collagen IV die in utero due to structural deficiencies in the basement membranes and certain mutations in this gene cause perinatal cerebral hemorrhage and porencephaly. Alternative splicing of this gene results in multiple transcript variants.
• Source: E. coli
• Species: Cattle
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 28.6 kDa; Accurate Molecular Mass: 29 kDa
• Protein length: Gly1445-Thr1669
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: COL4A1 collagen type IV alpha 1 chain [ Bos taurus (cattle) ]
• Official Symbol: COL4A1
• Synonyms: COL4A1; collagen type IV alpha 1 chain; collagen alpha-1(IV) chain; collagen, type IV, alpha 1
• Gene ID: 282191
• mRNA Refseq: NM_001166511
• Protein Refseq: NP_001159983