Recombinant Human COL4A1 protein, His & S-tagged

• Cat.No.: COL4A1-611H
• Product Overview: Recombinant Human COL4A1 aa. (His1444~Thr1669 (Accession # P02462)) fused with N-terminal His & S tag was produced in E. coli cells.

Specification

• Description: This gene encodes a type IV collagen alpha protein. Type IV collagen proteins are integral components of basement membranes. This gene shares a bidirectional promoter with a paralogous gene on the opposite strand. The protein consists of an amino-terminal 7S domain, a triple-helix forming collagenous domain, and a carboxy-terminal non-collagenous domain. It functions as part of a heterotrimer and interacts with other extracellular matrix components such as perlecans, proteoglycans, and laminins. In addition, proteolytic cleavage of the non-collagenous carboxy-terminal domain results in a biologically active fragment known as arresten, which has anti-angiogenic and tumor suppressor properties. Mutations in this gene cause porencephaly, cerebrovascular disease, and renal and muscular defects. Alternative splicing results in multiple transcript variants.
• Source: E. coli
• Species: Human
• Tag: His&S
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 30.7kDa
• Protein length: His1444~Thr1669
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 6.7.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: COL4A1 collagen type IV alpha 1 chain [ Homo sapiens (human) ]
• Official Symbol: COL4A1
• Synonyms: COL4A1; collagen type IV alpha 1 chain; BSVD; RATOR; collagen alpha-1(IV) chain; COL4A1 NC1 domain; arresten; collagen IV, alpha-1 polypeptide; collagen of basement membrane, alpha-1 chain
• Gene ID: 1282
• mRNA Refseq: NM_001303110.1
• Protein Refseq: NP_001290039.1
• UniProt ID: P02462