Active Recombinant Pig IL1A Protein (Ser113-Ser270), C-His tagged, Animal-free, Carrier-free

• Cat.No.: IL1A-203P
• Product Overview: Recombinant Pig IL1A Protein (Ser113-Ser270) with C-His tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Species: Pig
• Source: E.coli
• Tag: His
• Protein Length: Ser113-Ser270
• Description: Interleukin 1-alpha (IL-1α), also named hematopoietin 1, is a 17.65 kDa cytokine with 159 amino acid residues. IL-1α is mainly expressed in macrophages, neutrophils, epithelial cells, and endothelial cells. When IL-1α binds to the IL-1 receptor, It mediates a wide variety of biological processes such as cell division, angiogenesis, production of interleukin-2, and cellular sodium ion homeostasis. In addition, IL-1α also plays a critical role in immune responses, such as fever and inflammation, and triggers tumor necrosis factor-alpha.
• Form: Lyophilized
• Bio-activity: Measure by its ability to induce D10.G4.1 cells proliferation. The ED50 for this effect is < 50 pg/mL
• Molecular Mass: The protein has a calculated MW of 19.02 kDa.
  The protein migrates as 17-25 kDa under reducing condition (SDS-PAGE analysis).
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: >98% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: IL1A interleukin 1, alpha [ Sus scrofa ]
• Official Symbol: IL1A
• Synonyms: IL1A; interleukin 1, alpha; interleukin-1 alpha; IL-1 alpha; interleukin 1-alpha; IL-1alpha
• Gene ID: 397094
• mRNA Refseq: NM_214029
• Protein Refseq: NP_999194
• UniProt ID: P18430