Active Recombinant Human IL1A Protein

• Cat.No.: IL1A-02H
• Product Overview: Recombinant Human IL1A Protein (Ser113-Ala271) without tag was exoressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Protein Length: 13-271
• Description: The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease.
• Bio-activity: Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The ED50 for this effect is 1‑6 pg/mL.
• Molecular Mass: 18 kDa
• N-terminal Sequence Analysis: Ser113
• Endotoxin: < 0.10 EU/μg of the protein by the LAL method.
• Purity: > 97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Reconstitution: Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
• Shipping: The product is shipped at ambient temperature.

Gene Information

• Gene Name: IL1A interleukin 1 alpha [ Homo sapiens (human) ]
• Official Symbol: IL1A
• Synonyms: IL1A; interleukin 1 alpha; IL1; IL-1A; IL1F1; IL1-ALPHA; IL-1 alpha; interleukin-1 alpha; hematopoietin-1; preinterleukin 1 alpha; pro-interleukin-1-alpha
• Gene ID: 3552
• mRNA Refseq: NM_000575
• Protein Refseq: NP_000566
• MIM: 147760
• UniProt ID: P01583