Active Recombinant Mouse Il1a Protein

• Cat.No.: Il1a-099M
• Product Overview: Purified recombinant protein of Mouse interleukin 1 alpha (Il1a) (Ser115-Ser270) without tag was expressed in E.coli.

Specification

• Species: Mouse
• Source: E.coli
• Protein Length: 115-270 a.a.
• Description: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
• Bio-activity: The ED50 is 1- 5 pg/ml, corresponding to a specific activity of 1- 0.2 x 10^9 units/mg, determined by the dose dependent stimulation of D10S cells proliferation
• Molecular Mass: 18 kDa
• Endotoxin: Less than 0.01 ng per µg protein as determined by the LAL method
• Purity: >98%, as determined by Coomassie stained SDS-PAGE.
• Stability: Unopened vial can be stored between 2 centigrade and 8 centigrade for up to 2 weeks, at -20 centigrade for up to 6 months, or at -70 centigrade or below until the expiration date. Aliquots can be stored between 2 centigrade and 8 centigrade for up to one week and stored at -20 centigrade or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: Protein formulated and vialed at >50 ug/mL in specified buffer
• Storage Buffer: 10mM NaH2PO4, 150mM NaCl, pH7.2

Gene Information

• Gene Name: Il1a interleukin 1 alpha [ Mus musculus (house mouse) ]
• Official Symbol: Il1a
• Synonyms: Il1a; interleukin 1 alpha; Il-1a; interleukin-1 alpha; IL-1 alpha
• Gene ID: 16175
• mRNA Refseq: NM_010554
• Protein Refseq: NP_034684
• UniProt ID: P01582