Active Recombinant Mouse Wnt3a

• Cat.No.: Wnt3a-180M
• Product Overview: Recombinant Mouse Wnt3a was expressed in Insect cells.

Specification

• Species: Mouse
• Source: Insect Cells
• Tag: Non
• Protein Length: 328 a.a.
• Description: Wnt-3a signalling has been implicated in the control of differentiation of stem cells. Wnt proteins have been shown to regulate cell-to-cell interactions during embryogenesis, more specifically regulating mesoderm differentiation and osteogenesis. The Wnts have also been shown to have putative roles in the regulation of adult stem cells. Recombinant murine Wnt-3a is active in human cells. Recombinant murine Wnt-3a is a monomeric glycoprotein. Murine Wnt-3a migrates at an apparent molecular weight of approximately 35 kDa.
• Form: Colourless liquid.
• Bio-activity: ED50 of Wnt-3a is 12.5 - 25 ng/ml as determined by assay of mouse mammary epithelial cells (Eph4) with a TCF-fluorescent reporter.
• Molecular Mass: 35 kDa
• Endotoxin: Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
• Purity: >70%
• Stability: Wnt-3a is stable for at least 6 months when stored at -20ºC. Store long term at -80ºC. Withstands at least one freeze-thaw cycle. Aliquot before use.
• Storage: -20ºC
• Storage Buffer: Centrifuge vial before opening. Supplied at a concentration of 20 ug/ml in phosphate buffered saline with 1% BSA, 0.1M NaCl and 1% CHAPS.

Gene Information

• Gene Name: Wnt3a wingless-type MMTV integration site family, member 3A [ Mus musculus (house mouse) ]
• Official Symbol: Wnt3a
• Synonyms: Wnt3a; vt; Wnt-3a; wingless-type MMTV integration site family, member 3A; protein Wnt-3a; vestigial tail; wingless-related MMTV integration site 3A
• Gene ID: 22416
• mRNA Refseq: NM_009522
• Protein Refseq: NP_033548
• UniProt ID: P27467
• Chromosome Location: 11 B1.3; 11 37.17 cM
• Pathway: Basal cell carcinoma; Class B/2 (Secretin family receptors); Defective ACTH causes Obesity and Pro-opiomelanocortinin deficiency (POMCD)
• Function: frizzled binding; frizzled-2 binding; protein binding; protein domain specific binding