Recombinant Rat Wnt3a Protein, His-tagged

• Cat.No.: Wnt3a-586R
• Product Overview: Recombinant Rat Wnt3a Protein (Ser26-Lys359) with N-His tag was expressed in E. coli.

Specification

• Description: Predicted to enable several functions, including co-receptor binding activity; signaling receptor binding activity; and transcription coactivator activity. Predicted to be involved in several processes, including canonical Wnt signaling pathway involved in heart development; nervous system development; and positive regulation of macromolecule metabolic process. Predicted to act upstream of or within several processes, including animal organ development; chordate embryonic development; and positive regulation of macromolecule metabolic process. Predicted to be located in cell surface. Predicted to be part of Wnt-Frizzled-LRP5/6 complex. Predicted to be active in extracellular space and glutamatergic synapse. Biomarker of hypothyroidism. Orthologous to human WNT3A (Wnt family member 3A).
• Source: E. coli
• Species: Rat
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 41.0 kDa
  Accurate Molecular Mass: 41 kDa
• Protein length: Ser26-Lys359
• Purity: > 95%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 100mM NaHCO3, 500mM NaCl, pH8.3, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Wnt3a wingless-type MMTV integration site family, member 3A [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Wnt3a
• Synonyms: WNT3A; wingless-type MMTV integration site family, member 3A; protein Wnt-3a; wingless-related MMTV integration site 3A
• Gene ID: 303181
• mRNA Refseq: NM_001107005
• Protein Refseq: NP_001100475
• UniProt ID: F1M077