Active Recombinant Mouse Thymic Stromal Lymphopoietin, His-tagged

• Cat.No.: Tslp-202M
• Product Overview: Recombinant mouse TSLP produced ininsect cellis a 15.0 kDa protein consisting of 121 amino acid residues. It was expressed N‐terminal His‐Tagged amino acids Tyr20‐Glu140 of mTSLP.

Specification

• Cat. No.: Tslp-202M
• Description: Thymic Stromal Lymphopoietin (TSLP) is a member of the B cell‐stimulating factor family. Originally identified from the conditioned medium of a mouse thymic stromal cell line that promoted B‐cell development, TSLP is expressed in thymus, spleen, kidney, lung, and bone marrow. TSLP activity overlaps with that of IL‐7, playing a key role in the development of B cells, as well as stimulating the growth of thymocytes and T cells. TSLP stimulates monocytes to produce T cell‐attracting chemokines such as TARC (CCL17) and MDC (CCL22). TSLP also activates dendritic cellprimed CD4 T cells to produce Th2 cytokines, including IL‐4, IL‐5, IL‐13, and TNFα. This hematopoietic cytokine binds to a heterodimeric receptor complex consisting of the IL‐7R alpha chain (IL‐7Rα) and the TSLP‐specific chain (TSLPR). Binding induces activation of STAT3 and STAT5 via phosphorylation. Mouse TSLP shares approximately 43% amino acid sequence identity with human TSLP.
• Source: Insect cell.
• Molecular Mass: 15‐22 kDa on SDS‐PAGE due to glycosylation.
• Purity: Greater than 99%, as determined by SDS‐PAGE.
• Form: Sterile liquid; 20 mM NaH2PO4, 0.15M NaCl, pH 7.2 with 1.0% BSA. 0.22 μm filtered.
• Endotoxin Level: Less than 0.1 ng/ug cytokine as determined by the LAL assay.
• Bioactivity: The ED50 tested in a Nag8/7 cell proliferation assay was 0.15 ng/ml, corresponding to a specific activity of approximately 6x106 Units/mg.
• Applications Tested: This recombinant mouse TSLP has been tested in a Nag8/7 cell proliferation assay with an observed ED50 of 0.15 ng/ml, corresponding to a specific activity of approximately 6x106 Units/mg.
• Temperature Limitation: Store at less than or equal to ‐70°C.
• Handling Conditions: For best recovery, quick‐spin vial prior to opening. Use in a sterile environment.

Gene Information

• Gene Name: Tslp thymic stromal lymphopoietin [ Mus musculus ]
• Synonyms: Tslp; thymic stromal lymphopoietin; thymic stroma-derived lymphopoietin
• Gene ID: 53603
• mRNA Refseq: NM_021367
• Protein Refseq: NP_067342
• UniProt ID: Q9JIE6
• Chromosome Location: 18 B1; 18 11.0 cM
• Pathway: Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway
• Function: cytokine activity