Recombinant Human TSLP protein(R127A, R130S), hFc-tagged

• Cat.No.: TSLP-2335H
• Product Overview: Recombinant Human TSLP (NP_149024.1) (Met1-Gln159, R127A, R130S) was expressed in HEK293 with the fused Fc region of Human IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Human
• Tag: Fc
• Protein length: Met1-Gln159, R127A, R130S
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Immobilized Recombinant Human CRLF2 Protein (His Tag) at 2 μg/mL (100 μL/well) can bind Recombinant Human TSLP Protein (R127A, R130S, Fc Tag), the EC50 is 1.5-4.0 ng/mL.
• Molecular Mass: The recombinant Human TSLP consists of 369 amino acids and predicts a molecular mass of 41.50 kDa. It migrates as an approximately 46.22 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: TSLP thymic stromal lymphopoietin [ Homo sapiens ]
• Official Symbol: TSLP
• Synonyms: TSLP; thymic stromal lymphopoietin
• Gene ID: 85480
• mRNA Refseq: NM_033035
• Protein Refseq: NP_149024
• MIM: 607003
• UniProt ID: Q969D9