Active Recombinant Mouse Map4k1 Protein, GST-tagged

• Cat.No.: Map4k1-9520M
• Product Overview: Recombinant mouse Map4k1 (1-346) protein with an N-terminal GST tag was expressed by baculovirus in Sf9 insect cells.

Specification

• Description: HPK1 or Hematopoietic progenitor kinase 1 is a hematopoietic cell-restricted member of the Ste20 serine/threonine kinase super family. HPK1 is also known as mitogen-activated protein kinase kinase kinase kinase 1(MAP4K1). HPK1 is a tissue-specific upstream activator of the MEKK/JNK/SAPK signaling pathway. HPK1 diminishes T cell receptor (TCR) signaling activity and T cell proliferation by phosphorylating the adaptor protein SLP-76.
• Source: Insect cells
• Species: Mouse
• Tag: GST
• Bio-activity: The specific activity of HPK1 was determined to be 150.3 nmol/min/mg.
• Molecular Mass: ~65 kDa
• Purity: The purity of HPK1 was determined to be >90% by densitometry.
• Applications: Kinase Assay, Western Blot
• Stability: 1 year at -70 centigrade from date of shipment.
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.05 µg/µl
• Storage Buffer: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, and 25% glycerol.
• Protein length: 1-346 a.a.

Gene Information

• Gene Name: Map4k1
• Official Symbol: Map4k1 mitogen-activated protein kinase kinase kinase kinase 1 [ Mus musculus (house mouse) ]
• Synonyms: Map4k1; mHPK1; MEKKK1; Hpk1; mitogen-activated protein kinase kinase kinase kinase 1; mitogen-activated protein kinase kinase kinase kinase 1; HPK; MAPK/ERK kinase kinase kinase 1; MEK kinase kinase 1; MEKKK 1; hematopoietic progenitor kinase 1; EC 2.7.11.1
• Gene ID: 26411
• mRNA Refseq: NM_008279
• Protein Refseq: NP_032305
• UniProt ID: P70218