Recombinant Human mitogen-activated protein kinase kinase kinase kinase 1 Protein, His&Flag&StrepII tagged

• Cat.No.: MAP4K1-135H
• Product Overview: Recombinant human MAP4K1/HPK1 protein (Q92918) (Ser 324-Ser 457) with a Twin strep tag at the N-terminus and both His, Flag tag at the C-terminus was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: Flag&His&Twin Strep
• Protein Length: 324-457aa
• Description: Enables ATP binding activity and MAP kinase kinase kinase kinase activity. Involved in several processes, including JNK cascade; cellular response to phorbol 13-acetate 12-myristate; and protein phosphorylation. Located in membrane. Serine/threonine-protein kinase, which may play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway. May play a role in hematopoietic lineage decisions and growth regulation. Able to autophosphorylate 5. Together with CLNK, it enhances CD3-triggered activation of T-cells and subsequent IL2 production.
• Tag: N-Twin strep and C-His&Flag
• Molecular Mass: 21 kDa
• Endotoxin: Less than 1.0 EU/μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade. It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.
• Reconstitution: Reconstitute at 250 μg/mL in sterile water.

Gene Information

• Gene Name: MAP4K1 mitogen-activated protein kinase kinase kinase kinase 1 [ Homo sapiens (human) ]
• Official Symbol: MAP4K1
• Synonyms: MAP4K1; mitogen-activated protein kinase kinase kinase kinase 1; hematopoietic progenitor kinase 1; HPK1; MEKKK 1; MEK kinase kinase 1; MAPK/ERK kinase kinase kinase 1
• Gene ID: 11184
• mRNA Refseq: NM_001042600
• Protein Refseq: NP_001036065
• MIM: 601983
• UniProt ID: Q92918