Active Recombinant Mouse Interleukin-5

• Cat.No.: IL5-412M
• Product Overview: Interleukin-5 Mouse Recombinant produced in E.Coli is a dimeric, non-glycosylated polypeptide chain containing 2 x 113 amino acids forming a disulfide linked homodimer and having a molecular mass of 26.2 kDa. The Mouse IL-5 is purified by proprietary chromatographic techniques.

Specification

• Species: Mouse
• Source: Mouse
• Tag: Non
• Description: The protein encoded by this gene is a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. This cytokine is a main regulator of eosinopoiesis, eosinophil maturation and activation. The elevated production of this cytokine is reported to be related to asthma or hypereosinophilic syndromes. The receptor of this cytokine is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). This gene, together with those for interleukin 4 (IL4), interleukin 13 (IL13), and CSF2, form a cytokine gene cluster on chromosome 5. This cytokine, IL4, and IL13 are found to be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31.
• Form: Lyophilized from a concentrated (1mg/ml) solution containing 20mM Sodium Phosphate and 50mM NaCl pH-7.5.
• Purity: Greater than 97.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Bio-activity: The ED50 as determined by the dose-dependant stimulation of the proliferation of TF-1 cells was found to be 1.7ng/ml, corresponding to a specific activity of 588,235.3IU/mg.
• Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Solubility: It is recommended to reconstitute the lyophilized Mouse Interleikin-5 in sterile 18Mμ-cm H2O at a concentration of 100µg/ml, which can then be further diluted to other aqueous solutions.
• Amino acid sequence: MEIPMSTVVK ETLTQLSAHR ALLTSNETMR LPVPTHKNHQ LCIGEIFQGL DILKNQTVRG GTVEMLFQNL SLIKKYIDRQ KEKCGEERRR TRQFLDYLQE FLGVMSTEWA MEG.
• Storage: Lyophilized Mouse Interleukin-5 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Mouse IL5 should be stored at 4°C between 2-7 days and for future use below -18°C. Please avoid freeze thaw cycles.

Gene Information

• Gene Name: Il5 interleukin 5 [ Mus musculus ]
• Official Symbol: IL5
• Synonyms: interleukin-5; TRF; BCGF-II; OTTMUSP00000005818; B-cell growth factor II; T-cell replacing factor; cytotoxic T-lymphocyte inducer; eosinophil differentiation factor
• Gene ID: 16191
• mRNA Refseq: NM_010558
• Protein Refseq: NP_034688
• UniProt ID: P04401
• Chromosome Location: 11 A5/B1; 11 29.2 cM
• Pathway: Allograft rejection; Autoimmune thyroid disease; Fc epsilon RI signaling pathway; Intestinal immune network for IgA production; Jak-STAT signaling pathway
• Function: cytokine activity; growth factor activity; interleukin-5 receptor binding