Active Recombinant Canine IL5 Protein, His-tagged

• Cat.No.: IL5-456C
• Product Overview: Recombinant Canine IL5 (NP_001006951.1) (Met 1-Ser 134) was expressed, with a polyhistidine tag at the N-terminus.

Specification

• Species: Canine
• Source: Insect Cells
• Tag: His
• Protein Length: Met1-Ser134
• Description: Interleukin 5 (IL-5) is a member of the interleukin family with length of 115 amino acids. Interleukins are a group of cytokines (secreted proteins / signaling molecules) that were first seen to be expressed by white blood cells (leuk centigradeytes) and has been found in a wide variety of body cells. Interleukin 5 or IL-5 is produced by T helper-2 cells and mast cells. It helps to stimulate B cell growth and increase immunoglobulin secretion and is considered as a key mediator in eosinophil activation. Interleukin 5 (IL-5) has long been ass centigradeiated with several allergic diseases, including allergic rhinitis and asthma. Growth in the number of circulating, airway tissue, and induced sputum eosinophils have been observed in patients with these diseases. IL-5 also had something with the terminally differentiated granul centigradeyte eosinophils. IL-5 was originally found as an eosinophil colony stimulating factor. It has been proved to be a major regulator of eosinophil accumulation in tissues, and can modulate eosinophil behavior at every stage from maturation to survival.
• Predicted N Terminal: Val 22
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.5, 10% gly. Normally 5 %- 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 2-8 ng/mL.
• Molecular Mass: The recombinant canine IL5 consists of 123 amino acids and has a predicted molecular mass of 14.5 kDa. As a result of glycosylation, the apparent molecular mass of the canine IL5 is approximately 17-19 kDa in SDS-PAGE under reducing conditions
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 98 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at-70 centigrade. Store it under sterile conditions at-20 centigrade to-80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Official Symbol: IL5
• Synonyms: IL5;interleukin 5;interleukin-5;IL-5;T-cell replacing factor;TRF;eosinophil differentiation factor;interleukin 5 (colony-stimulating factor, eosinophil)