Recombinant Human Interleukin 5 (Colony-Stimulating Factor, Eosinophil)

• Cat.No.: IL5-528H
• Product Overview: Recombinant human IL-5 is a 26.5 kDa protein containing 232 amino acid residues.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: IL-5 is a potent T-cell derived factor that stimulates the growth of certain B cells and the differentiation of eosinophils.
• Biological Activity: The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is <0.15 ng/ml, corresponding to a specific activity of >6x106 units/mg.
• Molecular Weight: 26.5 kDa
• Form: Lyophilized (from 1 mg/ml in 0.4X PBS)
• Appearance: Lyophilized protein
• Endotoxin Level: <0.1 ng/μg
• Reconstitution: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1 mg/ml. The solution can be diluted into other buffered solutions or stored at -20°C for future use. For most in vitro applications, IL-5 exerts its biological activity in the concentration range of 0.1 to 10 ng/ml.
• Purity: >98% by SDS-PAGE and HPLC analysis
• Storage: The lyophilized IL-5 is best-stored desiccated below 0°C. Reconstituted IL-5 should be stored at working aliquots at -20°C. For long-term storage, it is recommended to add a carrier protein (0.1% HAS or BSA). Avoid freeze/thaw cycles.
• Pathways: Allograft rejection; Asthma; Autoimmune thyroid disease; Cytokine-cytokine receptor interaction; Fc epsilon RI signaling pathway; Hematopoietic cell lineage; Jak-STAT signaling pathway; cell receptor signaling pathway

Gene Information

• Gene Name: IL5 interleukin 5 (colony-stimulating factor, eosinophil) [ Homo sapiens ]
• Official Symbol: IL5
• Synonyms: IL5; interleukin 5 (colony-stimulating factor, eosinophil); EDF; TRF; IL-5; interleukin-5; OTTHUMP00000065830; T-cell replacing factor; B cell differentiation factor I; B-cell differentiation factor I; eosinophil differentiation factor; Eosinophil differentiation factor
• Gene ID: 3567
• mRNA Refseq: NM_000879
• Protein Refseq: NP_000870
• MIM: 147850
• UniProt ID: P05113
• Chromosome Location: 5q31.1
• Function: cytokine activity; growth factor activity; interleukin-4 receptor binding