Active Recombinant Mouse Growth Differentiation Factor 9

• Cat.No.: Gdf9-726M
• Product Overview: Recombinant Mouse Gdf9protein was expressed in CHO. Gly307-Arg441.

Specification

• Species: Mouse
• Source: CHO
• Tag: Non
• Protein Length: 307-441 a.a.
• Description: Gdf9 is a member ofthe transforming growth factor β (TGF-β) superfamily, and is an oocytesecreted paracrine factor essential for mammalian ovarian folliculogenesis.Mouse Gdf9 is synthesized as a 441 amino acid (aa) prepropeptide thatcontains a 29 aa signal sequence, a 277 aa propeptide, and a 135 aa maturechain). Residues 340-441 constitute a TGF-β like domain. In addition, thereis one potential site of N-linked glycosylation in the mature chain.
• Form: Lyophilized from a0.2 μm filtered solution in HCl with BSA as a carrier protein.
• N-terminalSequence: Gly307
• Molecular Weight: 15.6 kDa
• Activity: Measuredby its ability to induce Smad2 phosphorylation in P19 mouse embryonalcarcinoma cells. Mazerbourg, S. et al. (2004) Mol. Endocrinol. 18:653. 100ng/mL rmGdf9 can effectively induce Smad2 phosphorylation.
• Endotoxin Level: <1.0 EUper 1 μg of the protein by the LAL method.
• SDS-PAGE: 20 kDa,reducing conditions.
• Purity: >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: 100μg/mL in 4 mM HCl that containing 0.1% bovine serum albumin.
• Storage: Use a manualdefrost freezer and avoid repeated freeze-thaw cycles. 12 months from date ofreceipt, -20 to -70°C as supplied. 1 month, 2 to 8 °C under sterileconditions after reconstitution. 3 months, -20 to -70°C under sterileconditions after reconstitution.

Gene Information

• Gene Name: Gdf9 growth differentiationfactor 9 [ Mus musculus ]
• Official Symbol: Gdf9
• Synonyms: Gdf9; growthdifferentiation factor 9; Gdf-9; growth/differentiation factor 9
• Gene ID: 14566
• mRNA Refseq: NM_008110
• Protein Refseq: NP_032136
• UniProt ID: Q07105
• Chromosome Location: 11 B1.3; 11 29.0 cM
• Pathway: Ovarian InfertilityGenes; PluriNetWork
• Function: cytokine activity;growth factor activity