Recombinant Human Growth Differentiation Factor 9, His-tagged

• Cat.No.: GDF9-223H
• Product Overview: Recombinant Human Growth Differentiation Factor 9 encoding the mature human Growth differentiation factor 9 (Gln320-Arg454P) was recombined with his tag at the N-Terminus. That was expressed inE.Coli, and purified by Ni-NTA column. MW=14.9KD.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 320-454 a.a.
• Description: Growth/differentiation factor 9 is a protein that in humans is encoded by the GDF9 gene. Growth factors synthesized by ovarian somatic cells directly affect oocyte growth and function. Growth differentiation factor-9 (GDF9) is expressed in oocytes and is thought to be required for ovarian folliculogenesis. GDF9 is a member of the transforming growth factor-beta (TGFβ) superfamily. GDF9 plays an important role in the development of primary follicles in the ovary. It has a critical role in granulosa cell and theca cell growth, as well as in differentiation and maturation of the oocyte.
• Sequence: Human Growth differentiation factor (Gln³²⁰-Arg^454P)320GQETVSSELK KPLGPASFNL SEYFRQFLLP QNECELHDFR LSFSQLKWDN WIVAPHRYNP RYCKGDCPRA VGHRYGSPVH TMVQNIIYEK LDSSVPRPSC VPAKYSPLSV LTIEPDGSIA YKEYEDMIAT KCTCR 454.
• Purity: >95% in SDS gel.
• Formulation: Lyophilized 100 μg human Growth differentiation factor 9 in 50 μl of TBS (20 mM Tris, 50mM NaCl, pH8.0). Carry free.
• Application: Cell biology, ELISA.
• Reconstitution: Add 500 μl deionized water to the vial to prepare a working stock solution at 200μg/mL. Allow to set at least 30 minutes at 4°C, mix well.
• Storage: Store lyophilized protein at -20°C or -70°C. Lyophilized protein is stable for up to 6 months from date of receipt at -70°C. Upon reconstitution, this protein can be stored at for a few weeks or at -70°C in a manual defrost freezer for long term storage (six months). Aliquot reconstituted protein to avoid repeated freezing / thawing cycles.

Gene Information

• Gene Name: GDF9 growth differentiation factor 9 [ Homo sapiens ]
• Synonyms: GDF9; growth differentiation factor 9; OTTHUMP00000066213;GDF-9; Growth/differentiation factor 9
• Gene ID: 2661
• mRNA Refseq: NM_005260
• Protein Refseq: NP_005251
• MIM: 601918
• UniProt ID: O60383
• Chromosome Location: 5q31.1
• Function: cytokine activity; growth factor activity