Active Recombinant Human TNFSF12 Protein (Lys56-His249), C-His tagged, Animal-free, Carrier-free

• Cat.No.: TNFSF12-305H
• Product Overview: Recombinant Human TNFSF12 Protein (Lys56-His249) with C-His tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Description: TNF-related weak inducer of apoptosis (TWEAK) is a type II membrane protein of the tumor necrosis factor (TNF) family members, high levels expression in human brain, muscle, heart, spleen, and thymus. TWEAK is a 17.3 kDa protein containing 129 residues. TWEAK induce apoptosis through cognate receptor Fn14 activate caspase-8 and caspase-3, in HSC3 cells KATO-III gastric adenocarcinoma cells and IFN-γ-treated HT-29. TWEAK stimulate inflammatory cytokines in HT-29, A375 cells, WI-38 fibroblast sand astrocytes.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Lyophilized
• Bio-activity: Measure by its ability to induce proliferation in HUVEC cells. The ED50 for this effect is < 6 ng/mL.
• Molecular Mass: The protein has a calculated MW of 17.88 kDa.
  The protein migrates as 18 kDa under reducing condition (SDS-PAGE analysis).
• Protein length: Lys56-His249
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: >98% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 8.0.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: TNFSF12 tumor necrosis factor (ligand) superfamily, member 12 [ Homo sapiens (human) ]
• Official Symbol: TNFSF12
• Synonyms: TNFSF12; tumor necrosis factor (ligand) superfamily, member 12; tumor necrosis factor ligand superfamily member 12; APO3L; DR3LG; TWEAK; APO3 ligand; APO3/DR3 ligand; TNF-related WEAK inducer of apoptosis; MGC20669; MGC129581
• Gene ID: 8742
• mRNA Refseq: NM_003809
• Protein Refseq: NP_003800
• MIM: 602695
• UniProt ID: O43508