Active Recombinant Human Sp1 Transcription Factor

• Cat.No.: SP1-1157H

Specification

• Species: Human
• Source: HeLa
• Tag: Non
• Description: Sp1 was first detected in HeLa cells on the basis of its ability to activate the SV40 early promoter transcription. Subsequently it was shown to recognize and bind selectively to a GC-rich consensus sequence (GC-box: GGGCGG or CACCC) that presents in the promoter of several important cellular genes, including SV40 early, HIV-1, PDGF-B etc. Sp1 was the first transcription factor to be cloned and characterized. Analysis of structure and function has revealed that Sp1 can be separated into discrete functional domains. The DNAbinding domain consists of three zinc fingers that specifically bind to the GC-box element. Sp1 contains at least four separate transcriptional activation domains. Two of these domains are glutamine-rich, a wellcharacterized motif found in several other transcription factors. In addition to transcription, Sp1 function has been linked to cell growth, cancer, Huntington disease, and other disorders through transcriptional regulation or specific protein-protein interactions. The function of Sp1 can be regulated by phosphorylation and glycosylation. Natural Sp1 fraction was purified fromHeLacell nuclear extract by using wheat germ agglutinin affinity chromatography. Affinity purified Sp1 has been used for in vitro transcriptional activation, DNase I protection, and gel mobility shift assays. The Sp1 fraction is 80 - 90% pure and is devoid of other transcription factors.
• Form: Liquid. Supplied in 20 mM Tris-HCl pH 8.0, 100 mM KCl, 0.2 mM EDTA, 1 mM DTT, 20% glycerol.
• Purity: > 95% by SDS-PAGE.
• Activity: 50-100 ng are sufficient for a reconstituted transcription assay and 5-25 ng are sufficient for a gel mobility shift assay in a 20 μl reaction.
• Usage: For in vitro use only.
• Storage: Quality guaranteed for 12 months store at -80°C. Avoid freeze / thaw cycles.

Gene Information

• Gene Name: SP1 Sp1 transcription factor [ Homo sapiens ]
• Synonyms: SP1; Sp1 transcription factor; Sp1 transcription factor; specificity protein 1; TSFP1;Transcription factor Sp1
• Gene ID: 6667
• mRNA Refseq: NM_003109
• Protein Refseq: NP_003100
• MIM: 189906
• UniProt ID: P08047
• Chromosome Location: 12q13.1
• Pathway: Huntington"s disease; TGF-beta signaling pathway
• Function: RNA polymerase II transcription factor activity; double-stranded DNA binding; histone deacetylase binding; metal ion binding; promoter binding; protein C-terminus binding; protein homodimerization activity; transcription activator activity; transcription factor activity; zinc ion binding