Active Recombinant Human SP1, GST-tagged

• Cat.No.: SP1-8511H
• Product Overview: Recombinant Human SP1,fused with GST tag,was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: GST
• Description: Sp1 was first detected in HeLa cells on the basis of its ability to activate the SV40 early promoter transcription . Subsequently it was shown to recognize and bind selectively to a GC-rich consensus sequence (GC-box: GGGCGG or CACCC) that presents in the promoter of several important cellular genes, including SV40 early, HIV-1, PDGF-B etc. Sp1 was the first transcription factor to be cloned and . Analysis of structure and function has revealed that Sp1 can be separated into discrete functional domains. The DNA-binding domain consists of three zinc fingers that specifically bind to the GC-box element . Sp1 contains at least four separate transcriptional activation domains. Two of these domains are glutamine-rich, a well-characterized motif found in several other transcription factors . In addition to transcription, Sp1 function has been linked to cell growth, cancer, Huntington disease and other disorders through transcriptional regulation or specific protein-protein interactions. The function of Sp1 can be regulated by phosphorylation and glycosylation.
• Form: 20 mM Tris-Cl, pH 7.9, 20% Glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA
• Bio-activity: 1 unit equals 1 ng of purified protein. 100 units (ng) are sufficient for a protein-protein interaction assay.
• AA Sequence: NSVSAATLTP SSQAVTISSS GSQESGSQPV TSGTTISSAS LVSSQASSSS FFTNANSYST TTTTSNMGIM NFTTSGSSGT NSQGQTPQRV SGLQGSDALN IQQNQTSGGS LQAGQQKEGE QNQQTQQQQI LIQPQLVQGG QALQALQAAP LSGQTFTTQA ISQETLQNLQ LQAVPNSGPI IIRTPTVGPN GQVSWQTLQL QNLQVQNPQA QTITLAPMQG VSLGQTSSSN TTLTPIASAA SIPAGTVTVN AAQLSSMPGL QTINLSALGT SGIQVHPIQG LPLAIANAPG DHGAQLGLHG AGGDGIHDDT AGGEEGENSP DAQPQAGRRT RREACTCPYC KDSEGRGSGD P
• Purity: >95% as determined by SDS-PAGE
• Storage: -80 °C
• Concentration: 300 μg/ml
• Shipping: Dry Ice

Gene Information

• Gene Name: SP1 Sp1 transcription factor [ Homo sapiens ]
• Official Symbol: SP1
• Synonyms: SP1; Sp1 transcription factor; transcription factor Sp1; specificity protein 1
• Gene ID: 6667
• mRNA Refseq: NM_138473
• Protein Refseq: NP_612482
• MIM: 189906
• UniProt ID: P08047
• Chromosome Location: 12q13.1
• Pathway: Adipogenesis, organism-specific biosystem; Androgen Receptor Signaling Pathway, organism-specific biosystem; C-MYB transcription factor network, organism-specific biosystem; Direct p53 effectors, organism-specific biosystem; E2F transcription factor network, organism-specific biosystem; EGFR1 Signaling Pathway, organism-specific biosystem; Estrogen signaling pathway, organism-specific biosystem
• Function: DNA binding; HMG box domain binding; RNA polymerase II core promoter proximal region sequence-specific DNA binding; RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription; RNA polymerase II repressing transcription factor binding; bHLH transcription factor binding; double-stranded DNA binding; enhancer binding; histone acetyltransferase binding; histone deacetylase binding; metal ion binding; protein C-terminus binding; protein binding; protein homodimerization activity; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity; transcription factor binding; transcription regulatory region DNA binding; zinc ion binding