Active Recombinant Human NCOR1/HDAC3 Protein, GST-tagged

• Cat.No.: NCOR1-16H
• Product Overview: Recombinant Human NCOR1/HDAC3(NCOR1 amino acids 397-503) fused with GST tag was expressed in Insect cells.

Specification

• Description: Approximately 1:1 stoichiometric complex of recombinant human HDAC3 (histone deacetylase 3) with a GST fusion protein comprising the Deacetylase Activation Domain (DAD) of human NCOR1 (amino acids 397-503; SANT1 domain plus amino flanking residues). Produced by coexpression of HDAC3 with a GST-NCOR1(DAD) construct in an insect cell system, the complex displays ~100-fold higher deacetylase activity than isolated HDAC3 (based on equal weights HDAC3 assayed at a saturating concentration (100µM) of a fluorogenic peptide substrate p53 379-382, K(Ac)382).This HDAC3 preparation is active with various fluorogenic peptide substrates, including FLUOR DE LYS® Substrate and FLUOR DE LYS®-SIRT1 (p53 379-382, K(Ac)382).
• Source: Insect cells
• Species: Human
• Tag: GST
• Form: Liquid. In 50mM TRIS, pH 8.0, 138 mM NaCl and 10% glycerol.
• Bio-activity: ≥150 U/µg.
• Molecular Mass: 49/40 kDa
• Protein length: NCOR1 amino acids 397-503
• Purity: ≥90% (SDS-PAGE)
• Storage: Store at -80 centigrade

Gene Information

• Gene Name: NCOR1 nuclear receptor corepressor 1 [ Homo sapiens ]
• Official Symbol: NCOR1
• Synonyms: NCOR1; nuclear receptor corepressor 1; nuclear receptor co repressor 1; hCIT529I10; hN CoR; KIAA1047; MGC104216; N CoR; thyroid hormone and retinoic acid receptor associated corepressor 1; TRAC1; nuclear receptor co-repressor 1; thyroid hormone- and retinoic acid receptor-associated corepressor 1; N-CoR; N-CoR1; hN-CoR
• Gene ID: 9611
• mRNA Refseq: NM_001190438
• Protein Refseq: NP_001177367
• MIM: 600849
• UniProt ID: O75376