Recombinant Human NCOR1 protein, His & T7-tagged

• Cat.No.: NCOR1-1705H
• Product Overview: Recombinant Human NCOR1 aa. (Met1~Val284 (Accession # O75376)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: This gene encodes a protein that mediates ligand-independent transcription repression of thyroid-hormone and retinoic-acid receptors by promoting chromatin condensation and preventing access of the transcription machinery. It is part of a complex which also includes histone deacetylases and transcriptional regulators similar to the yeast protein Sin3p. This gene is located between the Charcot-Marie-Tooth and Smith-Magenis syndrome critical regions on chromosome 17. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 17 and 20.
• Source: E. coli
• Species: Human
• Tag: His&T7
• Form: Freeze-dried powder
• Molecular Mass: 40kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Met1~Val284
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >90%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Reconstitution: Reconstitute in sterile ddH2O.
• Isoelectric Point: 6.7

Gene Information

• Gene Name: NCOR1 nuclear receptor corepressor 1 [ Homo sapiens (human) ]
• Official Symbol: NCOR1
• Synonyms: NCOR1; nuclear receptor corepressor 1; N-CoR; TRAC1; N-CoR1; hN-CoR; PPP1R109; nuclear receptor corepressor 1; protein phosphatase 1, regulatory subunit 109; thyroid hormone- and retinoic acid receptor-associated corepressor 1
• Gene ID: 9611
• mRNA Refseq: NM_001190438.1
• Protein Refseq: NP_001177367.1
• UniProt ID: O75376