Active Recombinant Human LDLR protein, Fc-tagged

• Cat.No.: LDLR-0327H
• Product Overview: Recombinant Human LDLR protein(Ala22-Arg788), fused with Fc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 22-788 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris,100 mM Glycine,25 mM Arginine,150 mM NaCl,pH7.5 with trehalose as protectant.
• Bio-activity: Immobilized Human PCSK9 (D374Y), His Tag at 5 μg/mL (100 μL/well) can bind Human LDL R, Fc Tag with a linear range of 0.005-1.25 μg/mL.Immobilized Human LDL R, Fc Tag at 5 μg/mL (100 μL/well) can bind Human PCSK9 (D374Y), His Tag with a linear range of 10-78 ng/mL.
• Molecular Mass: This protein carries a human IgG1 Fc tag at the C-terminusThe protein has a calculated MW of 111.2 kDa. The protein migrates as 120-125 kDa and 140-150 kDa under reducing (R) condition, and 270-330 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Ala22-Arg788
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: LDLR low density lipoprotein receptor [ Homo sapiens ]
• Official Symbol: LDLR
• Synonyms: LDLR; low density lipoprotein receptor; low-density lipoprotein receptor; familial hypercholesterolemia; LDL receptor; low-density lipoprotein receptor class A domain-containing protein 3; FH; FHC; LDLCQ2
• Gene ID: 3949
• mRNA Refseq: NM_000527
• Protein Refseq: NP_000518
• MIM: 606945
• UniProt ID: P01130