Active Recombinant Human INS Protein

• Cat.No.: INS-53H
• Product Overview: Recombinant Human Insulin is a two chain polypeptide containing 51 amino acids and has a MW = 5.8 kDa

Specification

• Species: Human
• Source: Yeast
• Description: This gene encodes insulin, a peptide hormone that plays a vital role in the regulation of carbohydrate and lipid metabolism. After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified, including insulin-dependent diabetes mellitus, permanent neonatal diabetes diabetes mellitus, maturity-onset diabetes of the young type 10 and hyperproinsulinemia. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region.
• Bio-activity: 29 units/mg
• Molecular Mass: 5.8 kDa
• Endotoxin: < 1EU/mg
• Purity: >99.0% as determined by SEC-HPLC and SDS-PAGE.
• Storage: Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge vial prior to opening. Add sterile 0.01 N HCL to the vial to fully solubilize the protein to a concentration not less than 1 mg/ml, to be further diluted to the working concentration.

Gene Information

• Gene Name: INS insulin [ Homo sapiens (human) ]
• Official Symbol: INS
• Synonyms: IDDM; ILPR; IRDN; IDDM1; IDDM2; PNDM4; MODY10
• Gene ID: 3630
• mRNA Refseq: NM_000207.3
• Protein Refseq: NP_000198.1
• MIM: 176730
• UniProt ID: P01308