Active Recombinant Human HYAL2 Protein (Met21-Gly447), C-6×His-tagged

• Cat.No.: HYAL2-41H
• Product Overview: Recombinant Human Hyaluronidase 2/HYAL2 protein (Met21-Gly447) with a C-terminal 6-His tag was expressed in Chinese Hamster Ovary cell line.

Specification

• Species: Human
• Source: CHO
• Tag: His
• Protein Length: Met21-Gly447
• Description: This gene encodes a weak acid-active hyaluronidase. The encoded protein is similar in structure to other more active hyaluronidases. Hyaluronidases degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. Hyaluronan and fragments of hyaluronan are thought to be involved in cell proliferation, migration and differentiation. Although it was previously thought to be a lysosomal hyaluronidase that is active at a pH below 4, the encoded protein is likely a GPI-anchored cell surface protein. This hyaluronidase serves as a receptor for the oncogenic virus Jaagsiekte sheep retrovirus. The gene is one of several related genes in a region of chromosome 3p21.3 associated with tumor suppression. This gene encodes two alternatively spliced transcript variants which differ only in the 5' UTR.
• Bio-activity: Measured by its ability to digest Cy5-Labeled Hyaluronan (Low MW) to dp1-dp4.
  The majority of Cy5-Labeled Hyaluronan (Low MW) (2 pmol) is digested by 0.25 μg of Recombinant Human HYAL-2, as measured under the described conditions.
• Molecular Mass: Predicted Molecular Mass: 50 kDa
  SDS-PAGE: 53-58 kDa, under reducing conditions.
• N-terminal Sequence Analysis: Met21
• Endotoxin: <1.0 EU/μg of the protein by the LAL method.
• Purity: >95% by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  6 months from date of receipt, -20 to -70 centigrade as supplied.
  3 months, -20 to -70 centigrade under sterile conditions after opening.
• Storage Buffer: Supplied as a 0.2 μm filtered solution in Tris and NaCl.
• Shipping: The product is shipped with polar packs.

Gene Information

• Gene Name: HYAL2 hyaluronoglucosaminidase 2 [ Homo sapiens (human) ]
• Official Symbol: HYAL2
• Synonyms: HYAL2; hyaluronoglucosaminidase 2; hyaluronidase-2; hyaluronidase 2; LuCa 2; LUCA2; lysosomal hyaluronidase; PH 20 homolog; hyal-2; PH20 homolog; PH-20 homolog; lung carcinoma protein 2; hyaluronoglucosaminidase-2
• Gene ID: 8692
• mRNA Refseq: NM_003773
• Protein Refseq: NP_003764
• MIM: 603551
• UniProt ID: Q12891